If interested in sphagnum bogs you can find many things simply by rinsing some sphagnum and checking the rinsate under the scope. I also like to use a moist chamber for this. All sorts of interesting things can be found in a tuft/mat of sphagnum. Rotifers, tartigrades and numerous fungal spores. Leave a tuft of moss in a moist chamber with a small amount of water at the bottom and examine the water over time. These Helicoon spores are quite distinctive. Helicoon pleuriseptatum Beverwijk from Sphagnum washes Oct and Nov 2021 Lake Verde bog and Black Ck (small patch of Sphagnum near slow rivulet).
Conidia 40-60µm diax20-30µm height, flattened, 6-10 coils/spirals, dark brown.
Mycoportal includes 22 specimens from the UK, US, NZ and Russia (Siberia). Most from bog habitats.

Collected during the 11th International Mycological Congress in San Juan, Puerto Rico. #imc11

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Image #1:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 500
Color Corrected w/ X-Rite ColorChecker Passport
Image #2:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/16, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #3:
Canon EOS 6D + Canon MP-E 65mm f/2.8 1-5x + Yongnuo YN-14EX TTL LED Macro Ring Flash
1/180 sec, f/10, ISO 200
Color Corrected w/ X-Rite ColorChecker Passport
Image #4: Samsung Galaxy Note 4 + Dissecting Scope
Image #5:
100x – 3% KOH
Olympus CX30
Image #6:
400x – 3% KOH
Olympus CX30
Image #7:
1000x – 3% KOH
Olympus CX30
Image #8:
1000x – 3% KOH
Olympus CX30
Image #9:
1000x – 3% KOH
Olympus CX30
Image #10:
1000x – 3% KOH
Olympus CX30
Image #11:
1000x – 3% KOH
Olympus CX30
Image #12: habitat and host

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—MO custom fields—

Comments: see this species list for more observations of this fungus (or group of fungi), whose identity has evaded me for upwards of six years. in each example, the yellow stipe tissue turns deep purple in the presence of KOH. excited to not only to have a collection for sequencing and culturing, but for have received the provisional ID of Lindquistia — a xylariaceous anamorph — courtesy of IMA president, Dr. Keith Seifert, who I showed this to at the recent IMC in Puerto Rico. Dr. Seifert also possesses a portion of the collection, and I am working on gathering together more material from around the world.

Originally posted to Mushroom Observer on Jul. 27, 2018.

Permis de recherche scientifique Parc National d'Oka ;

PN0 2023-005

CG3045.
Érablière à caryer, chênes, hêtres. Au sol, grégaire.
Chapeau jusqu’à 4 cm. diam, viscidule.
Lames pourprées sur les jeunes spécimens, interveinées, sinuées.
Les lames pâlissent à maturité mais garde la couleur pourprée à l'arête.
Pied jaunâtre, strié, creux, longueur jusqu’à 6 cm.
Sporée blanche.
Récolté et déterminé par Yves Lamoureux.
Collectionné par Chantal Gauthier.

Non-stromatic, unitunicate, black pyrenomycetes fungi.
Small, hard perithecia up to 0.5 mm in diameter immersed in decorticated wood, with a well-developed neck. In a mixed forest.
Mature spores hyaline, with 3 septa, measure in H2O 25-30x5.9-6.4um

On deer dung tiny orange cup fungi with hyaline marginal hairs.
Asci 8-spored.
Ascospores ellipsoid, eguttulate, measured
*(18.7) 18.73 - 20.2 (20.4) × 9.3 - 10.3 (10.9) µm
Q = (1.8) 1.9 - 2 (2.1) ; N = 8
Me = 19.4 × 9.9 µm ; Qe = 2
Marginal hairs thick-walled, aseptate.

Growing abundantly on at least 3 well-rotted fallen hardwoods in close proximity. On the sides of the logs near where they meet the forest floor but not on the very bottoms. Salix nigra, Acer sacchariferum, Fagus grandifolia, Carya sp. and Carpinus caroliniana nearby as well as possibly some other hardwood species. Odor indistinct. Attached to wispy white rhizomorphs. Stems lateral. Caps finely fuzzy. Gills starting out pure white.

Small discomycete on decorticated hardwood next to Hormomyces aurantiacus. Apothecia up to 350um sessile.
8-spored asci with croziers, not sure about IKI, could be very weak blue, but strong blue after KOH. Asci measure in H2O
(43.2) 44.4 - 52.9 (54.4) × (6.7) 6.9 - 8.3 (8.6) µm
Q = (6.1) 6.13 - 6.5 (6.6) ; N = 6
Me = 48.7 × 7.7 µm ; Qe = 6.3
Ascospores ellipsoid, hyaline OCI=1, measure in H2O in asci
(7.2) 7.4 - 8.1 (8.3) × (2.8) 3.3 - 3.4 (3.8) µm
Q = (2) 2.1 - 2.4 (2.7) ; N = 6
Me = 7.7 × 3.3 µm ; Qe = 2.3
Paraphyses are cylindrical without VBs.
Marginal hairs smooth, slightly pigmented about 50-60x2.9-4.6um.
KOH negative.

Small cupulate fungi on Populus log. Apothecia are 0.4-0.7mm in diameter, sessile. Next to Scutellinia setosa.
Asci IKI+red, croziers(+).
Ascospores were still mostly in asci, hyaline, eguttulate, subfusiform, OCI=0-1, measure
(6.9) 7 - 9.3 (9.9) × (1.9) 2 - 2.5 (2.7) µm
Q = (2.6) 2.8 - 4.4 (4.6) ; N = 18
Me = 8.3 × 2.2 µm ; Qe = 3.7
Paraphyses are slightly lanceolate, without VBs.
Marginal hairs hyaline with many septa, 122x3.7um

I haven't seen this species before.

MRD0017

Station: Forêt d'Enseignement et de Recherche du Lac Duparquet

Lieu: Rapide-Danseur, Qc

Habitat : Forêt mixte (Sapin baumier et Peuplier Faux-tremble

Substrat : sous un tronc mort

Collectionneur : Mathias Rocheleau-Duplain

Déterminateur : Mathias Rocheleau-Duplain, Jonathan Mack et Jonathan Jensen-Lynch

MRD0040

1 janvier 2024
Pont-Rouge, Québec

Habitat : Corde de bois
Substrat : Buche d'Acer sp. (Érable)

Collectionneur : Mathias Rocheleau-Duplain

Taille du champignon : moins de 1 mm.

Mode de croissance : en colonie.

San Vicente Redwoods- Mixed hardwood/conifer forest that burned in the 2020 CZU fire
Growing on dead Quercus agrifolia leaves in an area with low severity burn, Quercus agrifolia, Arbutus menziesii and Sequoia sempervirens dominant
Sporocarops about the size of mustard seed, covered in white strongly filaments. Some sporocarps have pseudo-lamellae which express themselves as distant, smooth ridges (see in photo 1-3)

On basswood - Tilia americana leaf, on petiole, tiny cup fungi.
Apothecia 0.7-0.8mm in length.
Asci 8-spored, IKI+b, 36-46x5-6um, with croziers.
Ascospores eguttulate OCI=0, aseptate, measured
*(7) 7.3 - 8.2 (8.6) × (1.7) 1.8 - 2.2 (2.4) µm
Q = (3) 3.5 - 4.2 (4.8) ; N = 16
Me = 7.7 × 2 µm ; Qe = 3.8
Paraphyses cylindrical with long VBs.

MRD0015

Station : Forêt récréative Dudemaine

Habitat: Forêt mixte (épinette blanche, sapin baumier, pin gris, peuplier faux-tremble

Substrat : Au sol, sur la litière du sol de feuillus

Espèce inconnu

Collectionneur : Mathias Rocheleau-Duplain
Déterminateur :

Long necked Coelomycetes on a relatively young, recently fallen dead Acer rubrum.

Neck about 1mm tall at most, brownish-orange, progressively paler to hyaline at the tip. Conidia produced in a partially immersed pycnidia, conidiogesis apparently annelidic or possibly phiallidic, but clearly entheroblastic.

Conidia cylindrical, sometime very slightly clavate, hyaline, smooth and aseptate measuring 18-21 - 6-7.5(8) avg 19.3 - 7.

based on conidiogenesis and conidia, I am tempted to identify this as a member of the genus Cryptosporiopsis, but I am unable to get to an exact species in verkley monograph, C. coryli is probably close, but differ in its host. However, since I am not familiar with Coelomycetes, I might be completly wrong

growing out of what I believe to be a blueberry (possibly V. cestpitosum). Final image shows comparison with one that I think was growing out of a cranberry (V. oxycoccos - the lower one)

I’m really at a loss on this one. It’s odd that the sphagnum is white. There was some whitish mycelium or something on the sphagnum, but the orange parts aren’t elevated as illustrated for Mitrula. Also, this was cup-shaped, unlike the example Mitrula. Any thoughts?

On a wet ground, on a hiking path next to broadleaf forest tiny grey cup fungi. Open area and cups were growing between the grass.
Apothecia sessile, with marginal hairs, 1-2mm in diameter. Hymenium greyish, marginal hairs brown.
Asci 8-spored, operculate, IKI-, croziers(+), uniseriate.
Ascospores ellipsoid, eguttulate, uninucleate, smooth, hyaline, measured
(15.4) 15.5 - 17 (17.1) × (9.7) 10 - 11.28 (11.3) µm
Q = (1.4) 1.5 - 1.6 ; N = 13
Me = 16 × 10.6 µm ; Qe = 1.5
Paraphyses hyaline, filiform, septate, without VBs, straight.
Hairs with thick brown walls, septate, without roots, with two ends (one end goes up and the other shorter pointed down) like in *Trichophaeopsis bicuspis.

Melanospora arenaria L.Fisch. & Mont.
Habitat: lignicolous? (no other fungi evident in sample); large colony on underside of well-rotted conifer wood, wet subalpine forest (1800 m).
Ascomata: 1.0 - 1.1 mm total length; emerging from tufts of white mycelium at base, topped with a crown of hyaline setae that are arched to form a ‘cage’ joined at the top in a globular mass. Ascomata neck 800 - 900 µm long x 90 - 110 µm wide, composed of interwoven hyphae, yellowish-orange to pale brown, central cavity filled with spores. Setae 200-300 um long.
Spores: (11.7)12.4-13.8(14.7) x (9.6)9.9-11.2(11.9) µm; hyaline to dark brown, ellipsoidal, smooth-walled with a terminal germ pore at each end.

collected on rotten decorticated wood probably of Acer saccharum, in old mature forest

Description : Colonies on substrate effuse, greyish irregular, patches up to several mm in length (Picture 1).

Conidiophore arising from a slightly larger basal cell, erect, rarely branched, dematiaceous, except for apical cell (or occasionally cells), which tend to be much paler and slightly clavate, Lower part of conidiophore conspicuously ornamented with dark, flat, warts (Picture 6). Conidiophore up to 250µm in length and 3.2µm in width, very fragile (Picture 2-5).

Conidiongenous cells produced laterally, or much more rarely, at the apex of the conidiophore, Conidiogenous cells are dematiacous slightly bent and generally unbranched. Conidiogenesis from a single hyaline cup/bowl shaped collarette at the tip of conidiogenous cells (Picture 7-9) . Multiple conidia produced from a single collarette, the conidia agglutinate together into a sticky whitish mass

Conidia are hyaline asymetrical, somewhat bent or falcate, they are smooth and at least some have 1 septum at maturity. (Picture 10 - 11). conidia measure 10 - 13 x 1.5 - 2.5 µm. Conidia lack sheets and setulae (appendages)

Using the key in Seifert et al Genera of Hyphomycetes, I arrive to the genus Dictyochaetopsis, a genus that is not accepted by everyone. amongst the species in the genus D. glauconigra match this collection rather well, a description of D. glauconigra (as Menispora glauco-nigrum) is provided by Hughes in Kendrick in their review of the genus Menispora in 1963. The most notable difference is that the conidia or this collection might be slightly wider, and the conidiophore a bit shorter, but a good measurement of the latter was difficult due to their fragility. The only other species that I know that have the unusual ornamented conidiophore is the very similar D. apicalis (the type species of the genus), which differ mainly by its longer, 2-3 septate conidia.

@Keith_seifert

SPECIES NAME: Moellerodiscus advenulus (Phillips) Dumont

COLLECTIONS DATA: AC 2417, 2487. Auburn woodlot June 2020, 2021
SPECIES DESCRIPTION: ascomata translucent with orange-yellow to green "sphaerocysts/cells" on the stipe; asci blue in Lugols; spores 7-9x2-4um with gel appendages
NOTES: Seems to be widespread on Larix sp needles in June in the litter beneath trees. An excellent description is presented by Fallah & Shearer (2001) and the Freshwater Ascomycetes website. Some excellent photos are found on ASCO-SONNEBERG website as Calycina/Antinoa spores a bit larger and no apparent appendages. There is a R.F. Cain collection (Phialea advenula) near Norwich Ontario on Larix needles RF 2761 annotated by KP Dumont in the NYBG on Mycoportal. I assume the rest of the material is in TRTC. The best generic name for the species is not clear. The latest treatment discusses its placement in Lambertella (Zhao et al. 2016).

REFERENCES
Asco-Sonneberg website
Fallah & Shearer, 2001. Freshwater Ascomycetes: new or noteworthy species from north temperate lakes in Wisconsin. Mycologia 93: 566-602.
Freshwater Ascomycetes, http://fungi.life.illinois.edu/species_monographs
Zhao et al., 2016. Taxonomic re-evaluation of the genus Lambertella (Rutstroemiaceae, Helotiales) and allied stroma-forming fungi. Mycological Progress 15:1215-1228.

On Ulmus leaf next to Calycina ?populina.
Conidia hyaline, cylindrical, aseptate, measured
*(11.7) 12.4 - 14.7 (15.2) × (1.8) 1.9 - 2.2 (2.3) µm
Q = (5.3) 6 - 7.3 (7.4) ; N = 21
Me = 13.5 × 2.1 µm ; Qe = 6.6